HS003 – NEBuilder Cloning – pGADT7-TRIM69

Submitted by Hannah Sparks

Recombination of TRIM69 into pGADT7 occurred in this experiment in order to apply it to the Y2H assay later on. A recombination protocol was performed with a “vector only” sample as a control and a vector+insert sample. (Refer to B. Crane Experiment). Also wrote down the protocol for THP-1 cells to create a cDNA library, B. Crane completed the reaction.