HS003 – NEBuilder Cloning – pGADT7-TRIM69

Submitted by Hannah Sparks

Recombination of TRIM69 into pGADT7 occurred in this experiment in order to apply it to the Y2H assay later on. A recombination protocol was performed with a “vector only” sample as a control and a vector+insert sample. (Refer to B. Crane Experiment). Also wrote down the protocol for THP-1 cells to create a cDNA library, B. Crane completed the reaction. 

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s