HS006 – PCR of TRIM5, TRIM6, TRIM22, and TRIM56

Submitted by Hannah Sparks

This experiment was to perform PCR on TRIM 5 alpha, 6, 22, & 56 using the Q5 mastermix standard method. This experiment expects to have the TRIM DNA appear in bands in order to purify it later on. The gel shown in this experiment only yielded results for TRIM 5 and 6.   The PCR products will be cloned into pGADT7 Y2H plasmid.

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google+ photo

You are commenting using your Google+ account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

w

Connecting to %s