HS014 – TRIM5 and TRIM6 recombination with pGADT7 Using NEBuilder Hifi Enzyme Mix

Submitted by Hannah Sparks

A recombination of TRIM5a and TRIM6 into pGADT7 was performed in order to later before a bacterial transformation. B. Crane later on performed the bacterial transformation and no colonies formed, meaning that the transformation was unsuccessful. We will try again using purchased bacteria instead of our homemade bacteria.