BC003 – E. coli transformation with NEBuilder HiFi pGADT7-TRIM cloning reactions

Submitted by Brittnee Crane

LB Agar was mixed up and autoclaved; ampicillin was added and plates were poured for growing bacteria. A culture of Leishmania tarentolae and a culture of Vero cells were seeded and placed into the CO₂ incubator. Using homebrew bacteria, transformed bacteria with TRIM5α, TRIM6, TRIM22, TRIM31, TRIM56, and pGADT7-TRIM69. Some of the samples grew colonies, and all samples were placed in the 4°C in room 205.