BC004 – RT reactions of THP-1 cells treated with LPS

Submitted by Brittnee Crane

In a previous experiment, THP-1 cells were treated with different concentrations of LPS and the RNA was collected and purified using the Trizol Reagent protocol. The samples were then used in an RT reaction to turn the 24-hour RNA into cDNA. The cDNA will be used to complete PCRs of TRIM proteins, as they have been known to be expressed in THP-1 cells.