BC005 – E. coli transformations with pGADT7-TRIM cloning reactions

Submitted by Brittnee Crane

Bacterial transformations were completed using homebrew bacteria for cloned products: pGADT7-TRIM5α, pGADT7-TRIM6, pGADT7-TRIM69, pGADT7-empty(1), and pGADT7-empty(2). Unfortunately, no bacteria colonies grew for any of the samples. The transformations will be completed again using commercial bacteria.