HS017 – Plasmid miniprep of pGADT7-TRIM5a and TRIM6 colonies

Submitted by Hannah Sparks

B. Crane grew TRIM5a and TRIM6 colonies on AMP plates (refer to her lab notebook). Miniprep was performed on the pelleted cells in order to extract the DNA. This will be followed up with a digest using enzymes EcoRI and BamHI to screen for inserts of the expected sizes in the pGADT7 vector.