BC007 – Gel purification of TRIM6 variant 2 PCR product

Submitted by Brittnee Crane

Hannah’s amplicon mentioned in BC006 was run on a gel and was analyzed for the properly sized band. Landing at about 1,500 BP, the band showed promising results, so it was excised from the gel with a scalpel and put through the gel purification process. 5 microliters of the gel purified product were mixed with 2 microliters of purple loading dye and ran on a gel to estimate the concentration. I, unintentionally, skipped a crucial step in the gel purification process. The missed step lead to no visible results when run on a gel. The PCR will be completed again.

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