HS016 – TRIM5a, TRIM6, and TRIM69 PCR with plasmid DNA as template

Submitted by Hannah Sparks

PCR was performed on TRIM5a, TRIM6, and TRIM69. This was because we realized the primers we used was for cloning into pGBKT7 instead of pGADT7. We were going to take steps necessary to fix the primer mistake however we realized that due to the similarities of the primers for pGBKT7 and pGADT7, we will be able to proceed without taking additional steps. Therefore, we did not use these PCR products.