HS018 – Restriction screening of pGADT7-TRIM5a and pGADT7-TRIM6 clones

Submitted by Hannah Sparks

In this experiment, I digested plasmid from colonies of potential pGADT7-TRIM5a and pGADT7-TRIM6 clones with restriction enzymes EcoRI and BamHI. TRIM5a yielded expected band size. As expected, TRIM6 bands did not yield expected size, the size was smaller than the longest variant was supposed to be so we will perform PCR on Variant 2 to see if the primer anneals at the correct position.