BC009 – TRIM PCR gel electrophoresis

Submitted by Brittnee Crane

Made a 1% agarose gel using 120-mL of 1X TBE buffer, 1.2-g of agarose, and 12-μL of ethidium bromide. Added 5-μL of purple loading dye to each PCR (TRIM6, TRIM22, TRIM31, TRIM56, TRIM69) created in BC008 and loaded the samples into the gel. Ran the PCRs at 120 volts until the products passed the halfway mark on the gel, then analyzed the gel using a UV light. The desired products were not obtained, and all that was visible were primer dimers. The PCRs will be completed again with the addition of DMSO.

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