BC011 – Starting a culture of L. tarentolae in BHI without FBS

Submitted by Brittnee Crane

I seeded a culture of Leishmania tarentolae using an aliquot from the -80°C in room 205 of the CBB. The BHI broth used was half of the recommended concentration and didn’t contain any fetal bovine serum (FBS). In recent cultures, the parasites have grown way too rapidly and we are trying to better manage the rapid growth.


Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s