BC011 – Starting a culture of L. tarentolae in BHI without FBS

Submitted by Brittnee Crane

I seeded a culture of Leishmania tarentolae using an aliquot from the -80°C in room 205 of the CBB. The BHI broth used was half of the recommended concentration and didn’t contain any fetal bovine serum (FBS). In recent cultures, the parasites have grown way too rapidly and we are trying to better manage the rapid growth.

BC011

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