BC012 – Starting a new culture of THP-1 cells

Submitted by Brittnee Crane

I started a culture of THP-1 cells, using a frozen aliquot from the -80°C. The aliquot used, was from a batch that I grew and froze back, in what I found to be ideal conditions. My intentions are to treat the cells with LPS and 24 hours after the LPS treatment, to obtain the mRNA and purify it. The mRNA will be put through an RT reaction intended to produce cDNA. The cDNA will then be used in TRIM PCRs. Previous studies have shown that TRIM proteins are expressed at different levels in THP-1 cells.

 

BC012

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