BC013 – Starting a culture of HEK293 cells

Submitted by Brittnee Crane

I taught Luke Dominico how to start a culture of HEK-293 cells. A frozen aliquot, from the -80°C, was used to seed the culture. My intentions are to treat the cells with LPS and 24 hours after the LPS treatment, to obtain the mRNA and purify it. The mRNA will be put through an RT reaction intended to produce cDNA. The cDNA will then be used in PCRs.