HS020 – Continued attempts at slowing the growth rate of L. tarentolae

Submitted by Hannah Sparks

This experiment was to slow the growth rate of L. Tarentolae. Dr. Graff aspirated the media from one flask with 1X BHI and one flask with 1X BHI with 1% FBS and added media to both flasks again. The parasites grew remarkably fast again, so we decided to reduce the concentration of the BHI to half. Results showed that the parasites grew too quickly again, so we concluded that it would be best to start a new culture altogether. We may reduce the concentration of the BHI to 0.2X as an additional measure to slow the growth.