BC014 – TRIM PCR optimization using THP-1 +/- LPS cDNA samples

Submitted by Brittnee Crane

The cDNA treated with 5 ng/μL of LPS and that was mentioned in BC004a was used to make PCR mixtures for TRIM31, TRIM69, TRIM22, TRIM56, and TRIM6. Primers that were previously designed and ordered were used in the reaction, and two different reactions were made for each: one with 5% DMSO and one with 10% DMSO. The number of cycles was increased from 30 cycles to 35 cycles, and the extension time was increased from 1 minute to two minutes.