Submitted by Brittnee Crane
Made a 1% agarose gel using ~120-mL of 1xTBE buffer, ~1.2-g of agarose, and 12-μL of ethidium bromide. Two combs were placed in the gel box, so the gel was cut in half. Used only what was needed of the gel and stored the rest. Added 5-μL of purple loading dye to each PCR created in BC013 (TRIM31, TRIM69, TRIM22, TRIM56, and TRIM6) and loaded the samples into the gel. Ran the PCRs at 120 volts until the products passed the halfway mark on the gel, then analyzed the gel using a UV light.
Life and Biology 