BC019 – DNA gel with TRIM PCR samples from HS025

Submitted by Brittnee Crane

Gel electrophoresis was completed with the samples (TRIM5α, TRIM6, TRIM22, TRIM31, TRIM56, and TRIM69) that Hannah Sparks made in HS025. The Q5 Master Mix settings were used, but the annealing temperature was set to 57°C, the extension time was 2 minutes and the number of cycles was 37. Increasing the annealing temperature seems to be helping.

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