BC023 – Attempt at PCR optimization of TRIM69

Submitted by Brittnee Crane

Since TRIM69 is the reaction showing the most promise at the moment; four different PCR mixtures were made for TRIM69, that tested different conditions. The Q5 master mix settings were used in the thermocycler, but the annealing temperature was increased to 60°C, there were 37 cycles, and the extension time was set to 2 minutes.