HS021 – TRIM PCRs using THP-1 +/- LPS cDNA

Submitted by Hannah Sparks

In this experiment, PCR was performed to try to clone TRIM genes from the cDNA of LPS treated THP-1 cells. After performing gel electrophoresis, results showed that no bands appeared for the expected band size.  This could be due to the primers annealing to a different gene instead of the TRIM. Next time we perform PCR we will raise the annealing temperature to try to get the primers to have a higher specificity towards the TRIMs.