BC024 – pGADT7-TRIM6v2 plasmid miniprep and EcoRI/XhoI digest

Submitted by Brittnee Crane

Used the Mini Prep kit to obtain a purified plasmid from the culture of pGADT7-TRIM6 (variant 2) that was made in BC022. The plasmid was then digested with EcoR1 Hi-Fi and Xho1 [HS027] and ran on a gel for successful cloning verification.  Note: BamHI was not used to check for an insert because TRIM6v2 contains two BamHI sites.

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