BC026 – Splitting HEK293 Cells (293T)

Submitted by Brittnee Crane

The purpose of this experiment was to monitor the HEK293 cells, that were seeded in BC013, and get them to the proper confluency for RNA extraction using the TRIzol protocol and then followed by an RT-PCR. The result will yield cDNA from HEK293 cells that can be used for PCR reaction mixtures.

 

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