BC027 – HEK293 RNA Purification and Reverse Transcription

Submitted by Brittnee Crane

The purpose of this experiment was to put the HEK293 cells [BC026] through a TRIzol protocol and an RT-PCR reaction protocol. The two combined protocols will purify RNA from the cells, and then the RNA will be converted to cDNA. The HEK293 cDNA will be used in future experiments, as template DNA, to complete multiple PCR reaction mixtures.