BC028 – AH109 Yeast Prep for Transformations

Submitted by Brittnee Crane

The Matchmaker® Gold Yeast-Two-Hybrid System will be used in future experiments to detect possible protein-protein interactions with TRIM proteins and possibly other proteins (i.e. MAGE). The beginning of the protocol is starting a culture of yeast and getting it to an acceptable level for transforming plasmids into yeast. This experiment dealt with preparing the yeast for transformations.

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