Submitted by Brittnee Crane
After PCR and gel purification with the desired amplicon, TRIM69(variants C or D) was put through a recombination cloning reaction. The plasmid produced from the reaction was used to transform bacteria (E. coli). The bacteria that grew on the plate was used to seed a culture. The culture was purified and then digested. Following the digest completion, gel electrophoresis showed that the experiment was unsuccessful.
