BC031 – Cloning pGADT7-TRIM69 (Variant C or D) Attempt

Submitted by Brittnee Crane

After PCR and gel purification with the desired amplicon, TRIM69(variants C or D) was put through a recombination cloning reaction. The plasmid produced from the reaction was used to transform bacteria (E. coli). The bacteria that grew on the plate was used to seed a culture. The culture was purified and then digested. Following the digest completion, gel electrophoresis showed that the experiment was unsuccessful.


Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s