BC031 – Cloning pGADT7-TRIM69 (Variant C or D) Attempt

Submitted by Brittnee Crane

After PCR and gel purification with the desired amplicon, TRIM69(variants C or D) was put through a recombination cloning reaction. The plasmid produced from the reaction was used to transform bacteria (E. coli). The bacteria that grew on the plate was used to seed a culture. The culture was purified and then digested. Following the digest completion, gel electrophoresis showed that the experiment was unsuccessful.

 

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