HS028 – TRIM PCRs Using THP-1 (No LPS) cDNA

Submitted by Hannah Sparks

New TRIM primers were ordered so PCR was performed with them. The PCR performed TRIM 58 product from the cDNA from THP-1 cells treated with 0ng/uL of LPS. The PCR yielded TRIM 8 and 13 products from the Human cDNA (1:27). The next PCR was optimized with different amounts of DMSO, which produced only a band for TRIM 65 but at the incorrect size. All of the other TRIMs showed either no bands or streaking ones.

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