HS029 – Various Culturing Conditions for L. tarentolae

Submitted by Hannah Sparks

A new culture of L. tarentolae was seeded due to the culture results showed in HS026 that 1/2 X BHI of L. tarentolae died due to insufficient nutrients. This culture will be seeded in 5% FBS 1X BHI and 1% FBS 1X BHI. The media should provide sufficient nutrients for the L.Tarentolae to survive on. Once checked under the microscope, the parasites showed signs of being healthy. We expect the growth rate to be faster than the previous culture and for the parasites to be healthier.



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