HS033 – Preparing Broth and Agar for Yeast Experiments

Submitted by Hannah Sparks

Preparation of media for yeast transformations was performed on this day. YPDA plates and broth were made in order to have inventory for use at a later date. Drop out Agar was also made for yeast transformations with the use of the Matchmaker Gold Yeast Two-Hybrid System (box 2 of 2). Plates lacking “-leu”, “-trp”, “-leu-trp”, “leu-trp-his-ade,” were made for drop out agar plates.