HS034 – Quality Check of TRIM Plasmid Preps

Submitted by Hannah Sparks

Our box containing miniprep samples contained a lot of samples that we did not know if they were usable or not. In order to determine which samples we should keep in our inventory or toss out, we ran the samples on a gel and analyzed the band size to see if the samples contained the TRIM protein it was labeled as. We determined only three out of the eleven samples were good for future use. The rest of the samples were thrown away.

Note from Dr. Graff: This experiment ran undigested plasmid.  The bands on the gel represent supercoiled and nicked plasmids.  The plasmid stocks that were thrown away were of questionable quality and do not reflect whether they had the TRIM gene as this couldn’t be determined from the way the experiment was set up.

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