HS038 – pGADT7-TRIM13 Recombination Reaction for Cloning

Submitted by Hannah Sparks

Recombination was performed from the gel pure TRIM13 produced from the THP-1 (0ng/µl) cells, into EcoRI/BamHI digested pGADT7. The recombinations were accidentally left in the incubator for longer than the 1-hour incubation time. Therefore, this recombination was redone by K. Capp for bacterial transformation (refer to KC039).


Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s