BC032 – Digests for Subcloning TRIM5alpha and TRIM6v2 into pGBKT7

Submitted by Brittnee Crane

This experiment was designed to digest three different plasmids (pGBKT7-p53, pGADT7-TRIM5α, and pGADT7-TRIM6(var2)) that were intended for a ligation reaction. The purpose was to produce sticky ends so that the cloning would be efficient. The enzymes got mixed up and so it didn’t work the way that I wanted it to, but the experiment will be redone.

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s