BC032 – Digests for Subcloning TRIM5alpha and TRIM6v2 into pGBKT7

Submitted by Brittnee Crane

This experiment was designed to digest three different plasmids (pGBKT7-p53, pGADT7-TRIM5α, and pGADT7-TRIM6(var2)) that were intended for a ligation reaction. The purpose was to produce sticky ends so that the cloning would be efficient. The enzymes got mixed up and so it didn’t work the way that I wanted it to, but the experiment will be redone.

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