BC033 – PCR of Various TRIM Genes Using HEK293 cDNA

Submitted by Brittnee Crane

The HEK-293T cDNA mentioned in BC027 (sample 1) was used as template DNA in PCR mixtures with TRIM proteins: 2,8,13,14,21,22,25,27,28,31(variant 1 or 3), 31 (variant 2), 56,58, and 65. Some of the desired amplicons appeared (8,13,14,28,58, and 65), but the reaction size was very small and bands couldn’t be cut out. Some of the amplicons mentioned were within a streaking pattern.

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