BC035 – Yeast Transformation (Set 2)

Submitted by Brittnee Crane

The control plasmids required for using the yeast-two hybrid protocol (Matchmaker Gold) were transformed into yeast with the samples that were prepared in BC028. Two other plasmids (pGADT7-TRIM5α and pGADT7-TRIM6(variant 2)) were also transformed into yeast. Cultures were made for each transformation, which was then used to prepare glycerol stocks for future use.

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