BC037 – Quality Control Check of pcDNA3.1-HA-TRIM22 Plasmids

Submitted by Brittnee Crane

There were two sources of pcDNA3.1(+)-HA-TRIM22: a glycerol stock and a miniprep plasmid. The two samples were analyzed in hopes that one of them contained TRIM22 as expected. A culture from the glycerol stock was grown and put through a plasmid purification. A digest was performed on the new miniprep plasmid as well as the older one. Neither sample appeared to contain TRIM22. 

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