Submitted by Brittnee Crane
There were two sources of pcDNA3.1(+)-HA-TRIM22: a glycerol stock and a miniprep plasmid. The two samples were analyzed in hopes that one of them contained TRIM22 as expected. A culture from the glycerol stock was grown and put through a plasmid purification. A digest was performed on the new miniprep plasmid as well as the older one. Neither sample appeared to contain TRIM22.
Life and Biology 