BC039 – Transforming Bacteria with pcDNA-TRIM22, pcDNA31, and pCAGGS-TRIM69

Submitted by Brittnee Crane

The Whatman paper plasmids (pcDNA3.1(+)-HA-TRIM22, pcDNA3.1(+)-HA-TRIM31, and pCAGGS-HA-TRIM69) we received from Dr. Garcia-Sastre were transformed into bacteria. The bacterial colonies on the plates were used to start cultures.  The cultures were put through the plasmid purification process. pcDNA3.1(+)-TRIM31 showed a very low concentration that was unreliable, but the other two turned out great.  

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