BC041 – Checking insert size for pcDNA-HA-TRIM22 and pCAGGS-HA-TRIM69

Submitted by Brittnee Crane

The two different plasmids, pcDNA-HA-TRIM22 and pCAGGS-HA-TRIM69, produced in experiment BC039 were put through a double-digestion reaction (or sequential digest) to test whether an insert of the correct size was present. The insert is the area of interest, so this was somewhat of a check step before proceeding with PCR.  

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