BC042 – TRIM22 and TRIM69 PCRs Using Plasmids as Template

Submitted by Brittnee Crane

The purified plasmids produced in experiment BC039 were put through a dilution series and then used for PCR. pcDNA-HA-TRIM22 and pCAGGS-HA-TRIM69 were the two viable plasmids produced and the goal was to use the diluted plasmid to obtain an amplicon for TRIM22 and TRIM69. TRIM22 was unsuccessful, but PCR with TRIM69 appeared to pull out the 3 variants (A,BE, and CD) within TRIM69.  

 

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