Lab Meeting Notes – May 7, 2018

Lab Meeting

May 7, 2018

 

Sequencing

-getting plasmid-sequencing primer- ship through the mail

-analyze sequences (bioinformatics)

 

Archiving/Organizing

-glycerol stocks for all clones

-tables (charts/map for everything and they should be in their place)

-stockpiles of miniprep plasmids

Progress Report

wins for the team

-condensed summary for the week

-what you’re doing

 

Outreach, program development

– “fluff pieces”

-mini resumes

-techniques they have done

-youtube videos explaining cloning strategies

 

POLS videos (Riley)

-Prep videos through characterization of different viruses.

TRIM Review

-Write a review paper summary on the different TRIMS

-Literature Reviews about the TRIMs you are studying

-be able to talk about those papers and tell everyone about it.

 

Tasks for Team Leaders

  1. Perform literature searches on the TRIMs and MAGEs that you are studying.

-Come up with a computer file of different sequences, look at database, find splice variants, come up with cloning strategies to get rid of the extra sequences.

  1. Determine how you will keep track of hours worked and lab book pages logged.
  2. Look to see what PCR primer sets you have for your TRIMs/MAGEs. Make sure that there are primers designed for cloning by recombination into pGADT7 and pEGFP/mAzurite
  3. Start performing PCRs for cloning.  Then gel purify.  Then check gel.  Then recombination reactions.  Then transformations.  Then screening for inserts.
  4. Map out the splice variants for your TRIMs/MAGEs and design both general and variant-specific primers for qPCR.  Also design variant cloning strategies.

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