Week 2 – Hannah, Camille, and Kristine

For TRIM Proteins

Accomplishments:

  • Discovery of TRIM8 and TRIM13v3 gel purified PCR products in the freezer. Check gels confirmed the presence of these TRIMS in the correct place. Strong band of TRIM13v3 was present.
  • pTWIST-TRIM5a digest with NotI & BamHI and observation of TRIM5a at the correct size.

Failures:

  • pGADT7-TRIM5a digest showed TRIM5a in an unexpected spot with a curved band. When we referenced the sequence information for this plasmid, we decided to stop  using this plasmid for now and switched to pTWIST-TRIM5a.

Week 3 “To Do” List

  • PCR of pTWIST-TRIM5a
  • Gel pure of a TRIM8 band for use as template DNA for PCR.
  • Recombination of TRIM13v3 into pGADT7, pEGFP-C1, mAzurite, and pGBKT7
  • Bacterial Transformations of TRIM13v3 into pGADT7, pEGFP-C1, mAzurite, and GBKT7.
  • Finish up Glycerol Stock Archive Maps.

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