Submitted by Brittnee Crane for TRIM Proteins
Kurtiss observed as I finished wrapping up a Yeast two-hybrid experiment. I had already ran through the entire protocol, picked yeast colonies that grew on both double drop out and quadruple drop out agar plates with x-α-Gal (after mate and plate with a hcDNA yeast library), and streaked them out for isolation of a single yeast colonies. 15-cm agar plates were divided like a pizza and the sections were numbered. There were 9 colonies there were selected from plates, cultured for glycerol stocks and cultured for DNA purification. PCR was completed using designed primers and synthetic genes TRIM6, TRIM31 and TRIM69 for template.
Life and Biology 