Week 1 – Brittnee and Kurtiss

Submitted by Brittnee Crane for TRIM Proteins

Kurtiss observed as I finished wrapping up a Yeast two-hybrid experiment. I had already ran through the entire protocol, picked yeast colonies that grew on both double drop out and quadruple drop out agar plates with x-α-Gal (after mate and plate with a hcDNA yeast library), and streaked them out for isolation of a single yeast colonies. 15-cm agar plates were divided like a pizza and the sections were numbered. There were 9 colonies there were selected from plates, cultured for glycerol stocks and cultured for DNA purification. PCR was completed using designed primers and synthetic genes TRIM6, TRIM31 and TRIM69 for template.

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s