Week 3 – Hannah, Camille, and Kristine

For TRIM Proteins Research (TRIM5a, TRIM8, TRIM13, & TRIM58)

 Accomplishments: 

  •  Successful transformation of TRIM5a into mAzurite.
  •  Successful recombination/transformation of TRIM13 into pGADT7.
  •  PCR produced a band for TRIM5a for insertion into fluorescent plasmids.

 Failures:

  •  No colony formation from a recombination/transformation of TRIM13 into pEGFP-C1 or mAzurite.

 Week 4 “To Do” List 

  •  Get a PCR Product of TRIM8 for insertion into fluorescent plasmids.
  •  Miniprep of TRIM13 from insertion into pGADT7.
  •  Transform TRIM5a into mAzurite and pEGFP-C1.

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