For TRIM Proteins Research (TRIM5a, TRIM8, TRIM13, & TRIM58)
Accomplishments:
- Successful transformation of TRIM5a into mAzurite.
- Successful recombination/transformation of TRIM13 into pGADT7.
- PCR produced a band for TRIM5a for insertion into fluorescent plasmids.
Failures:
- No colony formation from a recombination/transformation of TRIM13 into pEGFP-C1 or mAzurite.
Week 4 “To Do” List
- Get a PCR Product of TRIM8 for insertion into fluorescent plasmids.
- Miniprep of TRIM13 from insertion into pGADT7.
- Transform TRIM5a into mAzurite and pEGFP-C1.