Brittnee Crane

Lab Book Entries

  • BC042 – TRIM22 and TRIM69 PCRs Using Plasmids as Template
  • BC041 – Checking insert size for pcDNA-HA-TRIM22 and pCAGGS-HA-TRIM69
  • BC040 – The Rise and Fall of the THP-1 Cell Empire
  • BC039 – Transforming Bacteria with pcDNA-TRIM22, pcDNA31, and pCAGGS-TRIM69
  • BC038 – Yeast Transformations – pGBKT7-p53, pGBKT7-Lam, and pGADT7-TRIM5alpha
  • BC037 – Quality Control Check of pcDNA3.1-HA-TRIM22 Plasmids
  • BC036 – Cloning TRIM6v2 into pGBKT7 via DNA Ligations
  • BC035 – Yeast Transformation (Set 2)
  • BC034 – THP-1 Cell Culture
  • BC033 – PCR of Various TRIM Genes Using HEK293 cDNA
  • BC032 – Digests for Subcloning TRIM5alpha and TRIM6v2 into pGBKT7
  • BC031 – Cloning pGADT7-TRIM69 (Variant C or D) Attempt
  • BC030 – Subcloning TRIM5alpha and TRIM6v2 from pGADT7 into pGBKT7
  • BC029 – Starting THP-1 Cultures from Frozen Stocks
  • BC028 – AH109 Yeast Prep for Transformations
  • BC027 – HEK293 RNA Purification and Reverse Transcription
  • BC026 – Splitting HEK293 Cells (293T)
  • BC025 – TRIM69 PCR optimization
  • BC024 – pGADT7-TRIM6v2 plasmid miniprep and EcoRI/XhoI digest
  • BC023 – Attempt at PCR optimization of TRIM69
  • BC022 – Master plate for pGADT7-TRIM6v2 cloning in BC020
  • BC021 – Making a glycerol stock for pGADT7-TRIM5alpha
  • BC020 – NEBuilder cloning reactions for pGADT7-TRIM6v2
  • BC019 – DNA gel with TRIM PCR samples from HS025
  • BC018 – Calculations and procedure for making yeast broth and agar
  • BC017 – Gel of PCR products from BC016
  • BC016 – Optimizing TRIM PCRs: Annealing temps, cycle number, and DMSO concentration
  • BC015 – Gel of BC014 PCR products
  • BC014 – TRIM PCR optimization using THP-1 +/- LPS cDNA samples
  • BC013 – Starting a culture of HEK293 cells
  • BC012 – Starting a new culture of THP-1 cells
  • BC011 – Starting a culture of L. tarentolae in BHI without FBS
  • BC010 – Running Hannah’s PCR product gel electrophoresis
  • BC009 – TRIM PCR gel electrophoresis
  • BC008 – PCR of various TRIMs using THP-1 (+/- LPS) cDNA
  • BC007 – Gel purification of TRIM6 variant 2 PCR product
  • BC006 – Transformation of commercial bacteria with pGADT7-TRIM5a and -TRIM6 NEBuilder reactions
  • BC005E. coli transformations with pGADT7-TRIM cloning reactions
  • BC004 – RT reactions of THP-1 cells treated with LPS
  • BC003 – E. coli transformation with NEBuilder HiFi pGADT7-TRIM cloning reactions
  • BC002 – TRIM31 PCR optimization
  • BC001 – Cloning TRIM31 and TRIM69 into pGADT7