Summer 2018 Weekly Reports
Lab Book Entries
- BC045 – Plasmid Minipreps – Y2H TRIMs and Controls
- BC044 – pGADT7 Digests to Prepare for Recombination Reactions
- BC042 – TRIM22 and TRIM69 PCRs Using Plasmids as Template
- BC041 – Checking insert size for pcDNA-HA-TRIM22 and pCAGGS-HA-TRIM69
- BC040 – The Rise and Fall of the THP-1 Cell Empire
- BC039 – Transforming Bacteria with pcDNA-TRIM22, pcDNA31, and pCAGGS-TRIM69
- BC038 – Yeast Transformations – pGBKT7-p53, pGBKT7-Lam, and pGADT7-TRIM5alpha
- BC037 – Quality Control Check of pcDNA3.1-HA-TRIM22 Plasmids
- BC036 – Cloning TRIM6v2 into pGBKT7 via DNA Ligations
- BC035 – Yeast Transformation (Set 2)
- BC034 – THP-1 Cell Culture
- BC033 – PCR of Various TRIM Genes Using HEK293 cDNA
- BC032 – Digests for Subcloning TRIM5alpha and TRIM6v2 into pGBKT7
- BC031 – Cloning pGADT7-TRIM69 (Variant C or D) Attempt
- BC030 – Subcloning TRIM5alpha and TRIM6v2 from pGADT7 into pGBKT7
- BC029 – Starting THP-1 Cultures from Frozen Stocks
- BC028 – AH109 Yeast Prep for Transformations
- BC027 – HEK293 RNA Purification and Reverse Transcription
- BC026 – Splitting HEK293 Cells (293T)
- BC025 – TRIM69 PCR optimization
- BC024 – pGADT7-TRIM6v2 plasmid miniprep and EcoRI/XhoI digest
- BC023 – Attempt at PCR optimization of TRIM69
- BC022 – Master plate for pGADT7-TRIM6v2 cloning in BC020
- BC021 – Making a glycerol stock for pGADT7-TRIM5alpha
- BC020 – NEBuilder cloning reactions for pGADT7-TRIM6v2
- BC019 – DNA gel with TRIM PCR samples from HS025
- BC018 – Calculations and procedure for making yeast broth and agar
- BC017 – Gel of PCR products from BC016
- BC016 – Optimizing TRIM PCRs: Annealing temps, cycle number, and DMSO concentration
- BC015 – Gel of BC014 PCR products
- BC014 – TRIM PCR optimization using THP-1 +/- LPS cDNA samples
- BC013 – Starting a culture of HEK293 cells
- BC012 – Starting a new culture of THP-1 cells
- BC011 – Starting a culture of L. tarentolae in BHI without FBS
- BC010 – Running Hannah’s PCR product gel electrophoresis
- BC009 – TRIM PCR gel electrophoresis
- BC008 – PCR of various TRIMs using THP-1 (+/- LPS) cDNA
- BC007 – Gel purification of TRIM6 variant 2 PCR product
- BC006 – Transformation of commercial bacteria with pGADT7-TRIM5a and -TRIM6 NEBuilder reactions
- BC005 – E. coli transformations with pGADT7-TRIM cloning reactions
- BC004 – RT reactions of THP-1 cells treated with LPS
- BC003 – E. coli transformation with NEBuilder HiFi pGADT7-TRIM cloning reactions
- BC002 – TRIM31 PCR optimization
- BC001 – Cloning TRIM31 and TRIM69 into pGADT7
