Brittnee Crane

Summer 2018 Weekly Reports

• Week 3 – Brittnee and Kurtiss
• Week 2 – Brittnee and Kurtiss
• Week 1 – Brittnee and Kurtiss

Lab Book Entries

• BC045 – Plasmid Minipreps – Y2H TRIMs and Controls
• BC044 – pGADT7 Digests to Prepare for Recombination Reactions
• BC042 – TRIM22 and TRIM69 PCRs Using Plasmids as Template
• BC041 – Checking insert size for pcDNA-HA-TRIM22 and pCAGGS-HA-TRIM69
• BC040 – The Rise and Fall of the THP-1 Cell Empire
• BC039 – Transforming Bacteria with pcDNA-TRIM22, pcDNA31, and pCAGGS-TRIM69
• BC038 – Yeast Transformations – pGBKT7-p53, pGBKT7-Lam, and pGADT7-TRIM5alpha
• BC037 – Quality Control Check of pcDNA3.1-HA-TRIM22 Plasmids
• BC036 – Cloning TRIM6v2 into pGBKT7 via DNA Ligations
• BC035 – Yeast Transformation (Set 2)
• BC034 – THP-1 Cell Culture
• BC033 – PCR of Various TRIM Genes Using HEK293 cDNA
• BC032 – Digests for Subcloning TRIM5alpha and TRIM6v2 into pGBKT7
• BC031 – Cloning pGADT7-TRIM69 (Variant C or D) Attempt
• BC030 – Subcloning TRIM5alpha and TRIM6v2 from pGADT7 into pGBKT7
• BC029 – Starting THP-1 Cultures from Frozen Stocks
• BC028 – AH109 Yeast Prep for Transformations
• BC027 – HEK293 RNA Purification and Reverse Transcription
• BC026 – Splitting HEK293 Cells (293T)
• BC025 – TRIM69 PCR optimization
• BC024 – pGADT7-TRIM6v2 plasmid miniprep and EcoRI/XhoI digest
• BC023 – Attempt at PCR optimization of TRIM69
• BC022 – Master plate for pGADT7-TRIM6v2 cloning in BC020
• BC021 – Making a glycerol stock for pGADT7-TRIM5alpha
• BC020 – NEBuilder cloning reactions for pGADT7-TRIM6v2
• BC019 – DNA gel with TRIM PCR samples from HS025
• BC018 – Calculations and procedure for making yeast broth and agar
• BC017 – Gel of PCR products from BC016
• BC016 – Optimizing TRIM PCRs: Annealing temps, cycle number, and DMSO concentration
• BC015 – Gel of BC014 PCR products
• BC014 – TRIM PCR optimization using THP-1 +/- LPS cDNA samples
• BC013 – Starting a culture of HEK293 cells
• BC012 – Starting a new culture of THP-1 cells
• BC011 – Starting a culture of L. tarentolae in BHI without FBS
• BC010 – Running Hannah’s PCR product gel electrophoresis
• BC009 – TRIM PCR gel electrophoresis
• BC008 – PCR of various TRIMs using THP-1 (+/- LPS) cDNA
• BC007 – Gel purification of TRIM6 variant 2 PCR product
• BC006 – Transformation of commercial bacteria with pGADT7-TRIM5a and -TRIM6 NEBuilder reactions
• BC005 – E. coli transformations with pGADT7-TRIM cloning reactions
• BC004 – RT reactions of THP-1 cells treated with LPS
• BC003 – E. coli transformation with NEBuilder HiFi pGADT7-TRIM cloning reactions
• BC002 – TRIM31 PCR optimization
• BC001 – Cloning TRIM31 and TRIM69 into pGADT7