Lab book posts
- HS038 – pGADT7-TRIM13 Recombination Reaction for Cloning
- HS037 – TRIM PCRs Using pcDNA Plasmids as Template
- HS036 – Restriction Mapping of pcDNA Plasmids with TRIM Gene Inserts
- HS035 – Check Gel of Gel-Purified TRIM13 PCR Product
- HS034 – Quality Check of TRIM Plasmid Preps
- HS033 – Preparing Broth and Agar for Yeast Experiments
- HS031 – Bacterial Transformations with pGADT7-TRIM69 Recombination Reactions
- HS030 – Preparing Stock Reagents for Yeast Transformations
- HS029 – Various Culturing Conditions for L. tarentolae
- HS028 – TRIM PCRs Using THP-1 (No LPS) cDNA
- HS027 – EcoRI/XhoI Digests for Subcloning TRIM5alpha and TRIM6v2 into pGBKT7
- HS026 – Leishmania tarentolae growth condition optimization
- HS025 – TRIM PCRs Using THP-1 cDNA as Template
- HS024 – Preparing Yeast Media
- HS023 – Mysterious swollen L. tarentolae (or budding yeast?)
- HS022 – DNA purification and check gel of TRIM6 variant 2 PCR
- HS021 – TRIM PCRs using THP-1 +/- LPS cDNA
- HS020 – Continued attempts at slowing the growth rate of L. tarentolae
- HS019 – TRIM6 variant 2 PCR using pcDNA-TRIM6 as template
- HS018 – Restriction screening of pGADT7-TRIM5a and pGADT7-TRIM6 clones
- HS017 – Plasmid miniprep of pGADT7-TRIM5a and TRIM6 colonies
- HS016 – TRIM5a, TRIM6, and TRIM69 PCR with plasmid DNA as template
- HS015 – More attempts to slow L. tarentolae growth rate
- HS014 – TRIM5 and TRIM6 recombination with pGADT7 Using NEBuilder Hifi Enzyme Mix
- HS013 – Reducing FBS to slow L. tarentolae growth
- HS012 – SwaI digest of pIR1HYG-mCherry for leishmania transfections
- HS011 – Evaluating parasites reseeded during late stationary growth phase
- HS010 – TRIM5a and TRIM6 PCR product gel purification
- HS009 – Continued monitoring of L. tarentolae growth
- HS008 – Plasmid Miniprep of TRIM6, TRIM31, and TRIM69
- HS007 – Leishmania tarentolae Growth Analysis
- HS006 – PCR of TRIM5, TRIM6, TRIM22, and TRIM56
- HS005 – Glycerol Stocks of Some TRIM Plasmids
- HS004 – Counting Leishmania with a Hemacytometer
- HS003 – NEBuilder Cloning – pGADT7-TRIM69
- HS002 – PCR Repeat – TRIM31 and TRIM69
- HS001 – TRIM31 and TRIM69 Cloning
