Hannah Sparks

20170714_091044

Lab book posts

  • HS038 – pGADT7-TRIM13 Recombination Reaction for Cloning
  • HS037 – TRIM PCRs Using pcDNA Plasmids as Template
  • HS036 – Restriction Mapping of pcDNA Plasmids with TRIM Gene Inserts
  • HS035 – Check Gel of Gel-Purified TRIM13 PCR Product
  • HS034 – Quality Check of TRIM Plasmid Preps
  • HS033 – Preparing Broth and Agar for Yeast Experiments
  • HS031 – Bacterial Transformations with pGADT7-TRIM69 Recombination Reactions
  • HS030 – Preparing Stock Reagents for Yeast Transformations
  • HS029 – Various Culturing Conditions for L. tarentolae
  • HS028 – TRIM PCRs Using THP-1 (No LPS) cDNA
  • HS027 – EcoRI/XhoI Digests for Subcloning TRIM5alpha and TRIM6v2 into pGBKT7
  • HS026 – Leishmania tarentolae growth condition optimization
  • HS025 – TRIM PCRs Using THP-1 cDNA as Template
  • HS024 – Preparing Yeast Media
  • HS023 – Mysterious swollen L. tarentolae (or budding yeast?)
  • HS022 – DNA purification and check gel of TRIM6 variant 2 PCR
  • HS021 – TRIM PCRs using THP-1 +/- LPS cDNA
  • HS020 – Continued attempts at slowing the growth rate of L. tarentolae
  • HS019 – TRIM6 variant 2 PCR using pcDNA-TRIM6 as template
  • HS018 – Restriction screening of pGADT7-TRIM5a and pGADT7-TRIM6 clones
  • HS017 – Plasmid miniprep of pGADT7-TRIM5a and TRIM6 colonies
  • HS016 – TRIM5a, TRIM6, and TRIM69 PCR with plasmid DNA as template
  • HS015 – More attempts to slow L. tarentolae growth rate
  • HS014 – TRIM5 and TRIM6 recombination with pGADT7 Using NEBuilder Hifi Enzyme Mix
  • HS013 – Reducing FBS to slow L. tarentolae growth
  • HS012 – SwaI digest of pIR1HYG-mCherry for leishmania transfections
  • HS011 – Evaluating parasites reseeded during late stationary growth phase
  • HS010 – TRIM5a and TRIM6 PCR product gel purification
  • HS009 – Continued monitoring of L. tarentolae growth
  • HS008 – Plasmid Miniprep of TRIM6, TRIM31, and TRIM69
  • HS007Leishmania tarentolae Growth Analysis
  • HS006 – PCR of TRIM5, TRIM6, TRIM22, and TRIM56
  • HS005 – Glycerol Stocks of Some TRIM Plasmids
  • HS004 – Counting Leishmania with a Hemacytometer
  • HS003 – NEBuilder Cloning – pGADT7-TRIM69
  • HS002 – PCR Repeat – TRIM31 and TRIM69
  • HS001 – TRIM31 and TRIM69 Cloning