HS011 – Evaluating parasites reseeded during late stationary growth phase

Submitted by Hannah Sparks

The parasites were still growing remarkably fast. Beginning to wonder if the FBS content in the BHI broth is too high. In this experiment, we reseeded at the same conditions to try to replicate the extreme growth that we saw. Another possible idea we came up with is to wait until they are completely in stationary phase before reseeding them so they will possibly grow slower. 

HS009 – Continued Monitoring of L. tarentolae Growth

Submitted by Hannah Sparks

This was the third count of L. tarentolae. It was expected to have a count of about 1e8 parasites/mL. Once the count confirmed that they were at the expected stage we decided to make a new sample with a lower count. Using calculations, we determined the amount of L. tarentolae from the current sample that we had to add to new media in order to decrease the count 1e5 parasites/mL. A count was redone in order to confirm that the count was decreased to the right amount, and it confirmed that it was. 

HS0013 – Reducing FBS to slow L. tarentolae growth

Submitted by Hannah Sparks

Parasites began to grow at an extremely fast rate. It was decided to decrease the amount of fetal bovine serum in the BHI media that they were growing in. Two new flasks were seeded, one with L. Tarentolae in BHI without FBS, and one with L. Tarentolae in BHI with 1% FBS. By decreasing the amount of fetal bovine serum, it is expected that their growth rate will slow down so we can catch them at a time that is optimal for transfection. 

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HS009 – Routine L. tarentolae culturing

Submitted by Hannah Sparks

This was the third count of L. tarentolae. It was expected to have a count of about 1e8 parasites/mL. Once the count confirmed that they were at the expected stage we decided to make a new sample with a lower count. Using calculations, we determined the amount of L. tarentolae from the current sample that we had to add to new media in order to decrease the count 2.5e5 parasites/mL. A count was redone in order to confirm that the count was decreased to the right amount, and it confirmed that it was.