For POLS Labs Plaque assays with bacteriophage involve overlaying "bottom agar" with "top agar" that contains bacteriophage-infected host bacteria. This video will show you the technique for doing this with a single tube of infected bacteria.  In practice, you will likely set up infections of several tubes of bacteria using serially diluted bacteriophage sample. The … Continue reading Phage Discovery Guide: Plaque Assay Protocol