For POLS Labs In the direct isolation protocol, the goal is to determine whether any bacteriophage can be detected in an environmental sample that is directly added to the host bacterial strain, such as Mycobacterium smegmatis.  This method is different than the enriched isolation protocol because no replication of the virus is allowed to occur before mixing … Continue reading Phage Discovery Guide: Direct Isolation Protocol

For POLS Labs There are two options described in the phage discovery guide for extracting bacteriophage from your environmental sample: enriched isolation (shown in this video) and direct isolation.  The enriched isolation is different from the direct isolation because the enriched isolation protocol is designed to add a step for bacteriophage replication before testing for infectious … Continue reading Phage Discovery Guide: Enriched Isolation Protocol