For biotechnology course The first transgenic mice had a growth hormone, somatotropin, expressed under the regulation of a metallothionein promoter. What supplement was added to the diet to induce somatotropin expression? In Greek mythology, what does a chimera look like? What does a chimeric mouse (usually) look like?  Would you expect to see a chimeric … Continue reading Transgenic Animals…Mostly Mice

For biotechnology Many questions were inspired by this CRISPR review. PAM stands for “protospacer adjacent motif”. What does this mean? Compare CRISPRi and CRISPRa. Which Cas9 variant would be useful for these approaches?  What Cas9 fusion protein would you create for CRISPRi?  CRISPRa? How would you set up your CRISPR-based experiment to preferentially use NHEJ … Continue reading Genome Editing with CRISPR

For Biotechnology course Describe Gregor Mendel’s experiments. What were some important conclusions from this work? Compare traditional breeding, mutation breeding, and transgenic approaches to creating new kinds of plants. Many plant cells are totipotent. In what ways can plant researchers make use of this trait? What does “Ti” stand for in Ti plasmid? What kind … Continue reading Plant culture and plant transgenics