For Discover Biology Lab (Background Information) It would probably be difficult to find a molecular biology laboratory that didn't have a freezer full of E. coli. Species that are commonly used in biology labs are called model organisms. You will find that there are people that can cite numerous reasons why one particular organism is … Continue reading Why work with Escherichia coli (E. coli)?
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Technique: Recombination Using NEBuilder HiFi DNA Assembly Master Mix
For Techniques Links Product site NEBuilder HiFi DNA Assembly Cloning Kit
My cloning attempt into a plasmid yielded colonies following transformation. Now what?
For Standard operating procedures Congratulations! But...before celebrating too much, make sure to check that your insert is what you think it is. Here are things you should work on now: Pick a colony or two from the plate and spot some of the bacteria onto another plate (aka masterplate) and shake the rest of the bacteria … Continue reading My cloning attempt into a plasmid yielded colonies following transformation. Now what?
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