For POLS Lab There's more than one way to isolate DNA from bacteriophages.  The SEA-PHAGES program recommends using a kit for doing the isolation.  The video shows the DNA extraction method that uses the kit.  However, at Montana Tech, students have been using a different protocol for many years. The quiz will rely heavily on … Continue reading DNA Extraction Protocol

For POLS https://youtu.be/GM9buhWJjlA

For POLS Lab Once you have taken your bacteriophage sample through two rounds of purification, it is time to amplify your clonal virus population.  This process involves first determining the titer of your purified phage.  (We will use a spot test of serially-diluted samples for this.)  Then, using the titer information, we will perform full-plate … Continue reading Protocol 6.3: Collecting Phage Lysate