Discover Biology Lab

Documents

  1. Discover Biology syllabus (Fall 2018)
  2. Lab book rubric
  3. Flow chart

Background Information

  1. Antiviral defense
  2. Project goals
  3. Why E. coli?
  4. Plasmid basics
  5. TRIM protein definition
  6. TRIM protein family functions
  7. Ubiquitin-proteasome system
  8. Ubiquitin enzyme cascade
  9. Antiviral signaling
  10. TRIM proteins in antiviral signaling
  11. Autophagy: bulk
  12. Autophagy: selective
  13. TRIM proteins in autophagy

Techniques

  1. Lab book advice
  2. Recombination
  3. Bacterial transformation
  4. Minipreps
  5. Restriction digests
  6. DNA gel electrophoresis (agarose gel)
  7. Gel analysis
  8. Restriction digest mapping
  9. Polymerase chain reaction (PCR)
  10. Yeast transformation
  11. Yeast selection: Plasmid uptake
  12. Yeast Selection: Reporter gene expression
  13. Yeast mating
  14. Mammalian cell transfection
  15. Inverted fluorescence microscopy
  16. Collecting lysate for immunoblots
  17. Protein gel electrophoresis (acrylamide gel)
  18. Protein transfer
  19. Immunoblotting

 

Information from Previous Semesters

Weekly Lab  Information and Videos